Journal: The Journal of Experimental Medicine

Article Title: Elevated levels of placental growth factor represent an adaptive host response in sepsis

doi: 10.1084/jem.20080398

Figure Lengend Snippet: Effect of PlGF deficiency on tissue mRNA/protein levels of inflammatory and hemostatic markers in a mouse model of endotoxemia. PLGF +/+ (WT) or PLGF −/− (KO) male mice were injected i.p. with or without 16 mg/kg LPS. (A) Shown are the results of quantitative real-time PCR analyses (mRNA copy number per 10 6 copies of 18S) of ICAM-1, VCAM-1, E-selectin, P-selectin, COX-2, and PAI-1 in the heart, lung and liver at 24 h. Data are expressed as means + SD of three independent experiments. *, P < 0.05; **, P < 0.01; and ***, P < 0.0001 compared with untreated controls (and where indicated between PlGF-deficient and wild-type mice). (B) Double immunofluorescence staining for activation markers and CD31 in the liver of wild-type mice treated in the absence (WT) or presence of 16 mg/kg LPS (WT/L) and PlGF −/− mice treated with 16 mg/kg LPS (PKO/L) at 24 h. (a) ICAM-1 (green) and CD31 (red). (b) VCAM-1 (green) and CD31 (red). (c) E-selectin (green) and CD31 (red). (d) P-selectin (green) and CD31 (red). (e) COX-2 (red) and CD31 (green). (f) PAI-1 (red) and CD31 (green). Bars: 132 μm; (insets) 42 μm.

Article Snippet: Immunohistochemistry was performed using the following primary antibodies: hamster monoclonal anti–mouse ICAM-1 (Serotec), rat monoclonal anti–mouse VCAM-1 antibody (BD Biosciences), rat monoclonal anti–mouse E-selectin antibody (BD Biosciences), rat monoclonal anti–mouse P-selectin antibody (Millipore), rabbit polyclonal anti–mouse COX-2 antibody (Cayman Chemical), and rabbit polyclonal anti–mouse PAI-1 antibody (Innovative Research Inc.).

Techniques: Injection, Real-time Polymerase Chain Reaction, Double Immunofluorescence Staining, Activation Assay